Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?
Joint Authors
Tsai, Yi-Chih
Chen, Chung-Chu
Li, Pei-Yi
Liu, Yin-Chang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
A random screening of human blood samples from 24 individuals of nonsmoker was conducted to examine the correlation between the oxidative DNA damage level of lymphocytes and the antioxidant capacity of serum or the base excision repair (BER) activity of lymphocytes.
The oxidative DNA damage level was measured with comet assay containing Fpg/Endo III cleavage, and the BER activity was estimated with a modified comet assay including nuclear extract of lymphocytes for enzymatic cleavage.
Antioxidant capacity was determined with trolox equivalent antioxidant capacity assay.
We found that though the endogenous DNA oxidation levels varied among the individuals, each individual level appeared to be steady for at least 1 month.
Our results indicate that the oxidative DNA damage level is insignificantly or weakly correlated with antioxidant capacity or BER activity, respectively.
However, lymphocytes from carriers of Helicobacter pylori (HP) or Hepatitis B virus (HBV) tend to give higher levels of oxidative DNA damage (P<0.05).
Though sera of this group of individuals show no particular tendency with reduced antioxidant capacity, the respective BER activities of lymphocytes are lower in average (P<0.05).
Thus, reduction of repair activity may be associated with the genotoxic effect of HP or HBV infection.
American Psychological Association (APA)
Tsai, Yi-Chih& Li, Pei-Yi& Chen, Chung-Chu& Liu, Yin-Chang. 2013. Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-456262
Modern Language Association (MLA)
Tsai, Yi-Chih…[et al.]. Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-456262
American Medical Association (AMA)
Tsai, Yi-Chih& Li, Pei-Yi& Chen, Chung-Chu& Liu, Yin-Chang. Is the Oxidative DNA Damage Level of Human Lymphocyte Correlated with the Antioxidant Capacity of Serum or the Base Excision Repair Activity of Lymphocyte?. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-456262
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456262