Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep
Joint Authors
Joana, Passos
Prada, Justina
Isabel, Pires
Paula, Rodrigues
Lígia, Bento
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Peripolar cells are granulated cells located in the vascular pole of the renal corpuscle.
Even though these cells have already been described, there are still many unknown histological and physiological characteristics.
We carried out histochemical and immunohistochemical analyses of peripolar cells in sheep and compared their number in both normal and injured kidneys, discriminating according to the age of the animal.
We tested HE, Toluidine Blue, PAS, and Masson's Trichrome stains to select the best stain for identification and quantification.
Masson Trichrome yielded the best results and was selected for this purpose.
We identified the cells by the presence of cytoplasmatic granules and by their position in the vascular pole.
We found no statistically significant association between the number of peripolar cells and the age of the animal or the occurrence of lesions.
In the immunohistochemical analysis, we found that the cells were positive to α-smooth muscle actin and less consistently positive to NSE and S100 protein.
Chromogranin A, cyclooxygenase-2, AE1/AE3, and Wide Spectrum Cytokeratin and desmin yielded negative results.
We conclude that although there was evidence of a contractile function, there was no evidence to support that peripolar cells have either a neuroendocrine or an epithelial nature.
American Psychological Association (APA)
Joana, Passos& Prada, Justina& Lígia, Bento& Paula, Rodrigues& Isabel, Pires. 2013. Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep. Journal of Histology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456266
Modern Language Association (MLA)
Joana, Passos…[et al.]. Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep. Journal of Histology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-456266
American Medical Association (AMA)
Joana, Passos& Prada, Justina& Lígia, Bento& Paula, Rodrigues& Isabel, Pires. Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep. Journal of Histology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456266