Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract from Nigella sativa Seed in Rat Aorta : The Roles of Ca2+ and K+ Channels
Joint Authors
Fereidouni, Elahe
Mousavi, Seyed Mojtaba
Niazmand, Saeed
Mahmoudabady, Maryam
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
The aim of this study was to elucidate the mechanism(s) responsible for the vasorelaxant effect of Nigella sativa (N.
sativa).
Methods.
The activity of different concentrations of N.
sativa extract was evaluated on contractile responses of isolated aorta to KCl and phenylephrine (PE).
Results.
The extract (2–14 mg/mL) induced a concentration dependent relaxation both in endothelium-intact and endothelium-denuded aortic rings precontracted by PE (10−6 M) and KCl (6 × 10−2 M).
Extract reduced PE- and KCl-induced contractions in presence of cumulative concentrations of calcium (10−5–10−2 M) significantly.
L-NAME and indomethacin had no effect on vasorelaxation effect of extract in PE-induced contraction.
Diltiazem and heparin reduced significantly this vasorelaxation at a concentration of 14 mg/mL of extract; however, N.
sativa-induced relaxation was not affected by ruthenium red.
Tetraethylammonium chloride reduced the extract-induced relaxation in concentrations of 2–6 mg/mL of extract significantly but glibenclamide reduced this relaxative effect in all concentrations of extract.
Conclusions.
The inhibitory effect of N.
sativa seed extract on the contraction induced by PE and KCl was endothelium-independent.
This relaxation was mediated mainly through the inhibition of Ca2+ and KATP channels and also intracellular calcium release.
American Psychological Association (APA)
Niazmand, Saeed& Fereidouni, Elahe& Mahmoudabady, Maryam& Mousavi, Seyed Mojtaba. 2014. Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract from Nigella sativa Seed in Rat Aorta : The Roles of Ca2+ and K+ Channels. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-457058
Modern Language Association (MLA)
Niazmand, Saeed…[et al.]. Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract from Nigella sativa Seed in Rat Aorta : The Roles of Ca2+ and K+ Channels. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-457058
American Medical Association (AMA)
Niazmand, Saeed& Fereidouni, Elahe& Mahmoudabady, Maryam& Mousavi, Seyed Mojtaba. Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract from Nigella sativa Seed in Rat Aorta : The Roles of Ca2+ and K+ Channels. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-457058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457058