Two Distantly Spaced Basic Patches in the Flexible Domain of Huntingtin-Interacting Protein 1 (HIP1) Are Essential for the Binding of Clathrin Light Chain
Joint Authors
Illingworth, Melissa
Niu, Qian
Gonzalez, Claire
Ybe, Joel A.
Clegg, Mary E.
Source
Research Letters in Biochemistry
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-04-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The interaction between HIP family proteins (HIP1 and HIP12/1R) and clathrin is fundamental to endocytosis.
We used circular dichroism (CD) to study the stability of an HIP1 subfragment (aa468-530) that is splayed open.
CD thermal melts show HIP1 468-530 is only stable at low temperatures, but this HIP1 fragment contains a structural unit that does not melt out even at 83C∘.
We then created HIP1 mutants to probe our hypothesis that a short hydrophobic path in the opened region is the binding site for clathrin light chain.
We found that the binding of hub/LCb was sensitive to mutating two distantly separated basic residues (K474 and K494).
The basic patches marked by K474 and K494 are conserved in HIP12/1R.
The lack of conservation in sla2p (S.
cerevisiae), HIP1 from D.
melanogaster, and HIP1 homolog ZK370.3 from C.
elegans implies the binding of HIP1 and HIP1 homologs to clathrin light chain may be different in these organisms.
American Psychological Association (APA)
Ybe, Joel A.& Clegg, Mary E.& Illingworth, Melissa& Gonzalez, Claire& Niu, Qian. 2009. Two Distantly Spaced Basic Patches in the Flexible Domain of Huntingtin-Interacting Protein 1 (HIP1) Are Essential for the Binding of Clathrin Light Chain. Research Letters in Biochemistry،Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-457831
Modern Language Association (MLA)
Ybe, Joel A.…[et al.]. Two Distantly Spaced Basic Patches in the Flexible Domain of Huntingtin-Interacting Protein 1 (HIP1) Are Essential for the Binding of Clathrin Light Chain. Research Letters in Biochemistry No. 2009 (2009), pp.1-5.
https://search.emarefa.net/detail/BIM-457831
American Medical Association (AMA)
Ybe, Joel A.& Clegg, Mary E.& Illingworth, Melissa& Gonzalez, Claire& Niu, Qian. Two Distantly Spaced Basic Patches in the Flexible Domain of Huntingtin-Interacting Protein 1 (HIP1) Are Essential for the Binding of Clathrin Light Chain. Research Letters in Biochemistry. 2009. Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-457831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457831