CD73 Is Critical for the Resolution of Murine Colonic Inflammation
Joint Authors
Mueller, Cynthia
Czopik, Agnieszka
Mahamed, Deeqa A.
Bynoe, Margaret S.
Mills, Jeffrey H.
Waickman, Adam T.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
CD73 is a glycosyl-phosphatidylinositol-(GPI-) linked membrane protein that catalyzes the extracellular dephosphorylation of adenosine monophosphate (AMP) to adenosine.
Adenosine is a negative regulator of inflammation and prevents excessive cellular damage.
We investigated the role of extracellular adenosine in the intestinal mucosa during the development of Dextran-Sulfate-Sodium-(DSS-)salt-induced colitis in mice that lack CD73 (CD73−/−) and are unable to synthesize extracellular adenosine.
We have found that, compared to wild-type (WT) mice, CD73−/− mice are highly susceptible to DSS-induced colitis.
CD73−/− mice exhibit pronounced weight loss, slower weight recovery, an increase in gut permeability, a decrease in expression of tight junctional adhesion molecules, as well as unresolved inflammation following the removal of DSS.
Moreover, colonic epithelia in CD73−/− mice exhibited increased TLR9 expression, high levels of IL-1β and TNF-α, and constitutive activation of NF-κB.
We conclude that CD73 expression in the colon is critical for regulating the magnitude and the resolution of colonic immune responses.
American Psychological Association (APA)
Bynoe, Margaret S.& Waickman, Adam T.& Mahamed, Deeqa A.& Mueller, Cynthia& Mills, Jeffrey H.& Czopik, Agnieszka. 2012. CD73 Is Critical for the Resolution of Murine Colonic Inflammation. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-458317
Modern Language Association (MLA)
Bynoe, Margaret S.…[et al.]. CD73 Is Critical for the Resolution of Murine Colonic Inflammation. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-458317
American Medical Association (AMA)
Bynoe, Margaret S.& Waickman, Adam T.& Mahamed, Deeqa A.& Mueller, Cynthia& Mills, Jeffrey H.& Czopik, Agnieszka. CD73 Is Critical for the Resolution of Murine Colonic Inflammation. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-458317
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458317