Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area
Joint Authors
Song, Wei
Chen, Guoping
Kawazoe, Naoki
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
Micropatterning technology is a highly advantageous approach for directly assessing and comparing the effects of different factors on stem cell functions.
In this study, poly(vinyl alcohol)- (PVA-) micropatterned polystyrene surfaces were prepared using photoreactive PVA and ultraviolet photolithography with a photomask.
The micropatterned surface was suitable for single-cell array formation and long-term cell culture due to the nanometer thickness of nonadhesive PVA layer.
Different degrees of cell spreading with the same cell shape were established by adjusting the sizes of circular, cell-adhesive polystyrene micropatterns.
Cell spreading and differentiation of mesenchymal stem cells (MSCs) on the micropatterns were investigated at the single-cell level.
The assembly and organization of the cytoskeleton were regulated by the degree of cell spreading.
Individual MSCs on large circular micropatterns exhibited a more highly ordered arrangement of actin filaments than did those on the small circular micropatterns.
Furthermore, the differentiation of MSCs was dependent on the degree of cell spreading.
Increased cell spreading facilitated the osteogenic differentiation but suppressed the adipogenic differentiation of MSCs.
This micropatterning method is valuable for stem cell research in tissue engineering and regenerative medicine.
American Psychological Association (APA)
Song, Wei& Kawazoe, Naoki& Chen, Guoping. 2011. Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area. Journal of Nanomaterials،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-458721
Modern Language Association (MLA)
Song, Wei…[et al.]. Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area. Journal of Nanomaterials No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-458721
American Medical Association (AMA)
Song, Wei& Kawazoe, Naoki& Chen, Guoping. Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area. Journal of Nanomaterials. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-458721
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458721