![](/images/graphics-bg.png)
A Splice Variant of ASC Regulates IL-1β Release and Aggregates Differently from Intact ASC
Joint Authors
Sagara, Junji
Taniguchi, Shun'ichiro
Nakamura, Akihiro
Matsushita, Kazuhiko
Itano, Naoki
Takeoka, Michiko
Kurose, Yuka
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-15
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The apoptosis-associated speck-like protein containing a caspase recruit domain (ASC) is involved in apoptosis and innate immunity and is a major adaptor molecule responsible for procaspase-1 activation.
ASC mRNA is encoded by three exons: exons 1 and 3 encode a pyrin domain (PYD) and caspase recruit domain (CARD), respectively, and exon 2 encodes a proline and glycine-rich (PGR) domain.
Here, we identified a variant ASC protein (vASC) lacking the PGR domain that was smaller than full length ASC (fASC) derived from fully transcribed mRNA and searched for differences in biochemical and biological nature.
Both fASC and vASC were found to activate procaspase-1 to a similar degree, but the efficiency of IL-1β excretion was significantly higher for vASC.
There was also a marked structural difference observed in the fibrous aggregates formed by fASC and vASC.
These results suggest that although the PGR domain is dispensable for procaspase-1 activation, it plays an important role in the regulation of the molecular structure and activity of ASC.
American Psychological Association (APA)
Matsushita, Kazuhiko& Takeoka, Michiko& Sagara, Junji& Itano, Naoki& Kurose, Yuka& Nakamura, Akihiro…[et al.]. 2009. A Splice Variant of ASC Regulates IL-1β Release and Aggregates Differently from Intact ASC. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-460561
Modern Language Association (MLA)
Matsushita, Kazuhiko…[et al.]. A Splice Variant of ASC Regulates IL-1β Release and Aggregates Differently from Intact ASC. Mediators of Inflammation No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-460561
American Medical Association (AMA)
Matsushita, Kazuhiko& Takeoka, Michiko& Sagara, Junji& Itano, Naoki& Kurose, Yuka& Nakamura, Akihiro…[et al.]. A Splice Variant of ASC Regulates IL-1β Release and Aggregates Differently from Intact ASC. Mediators of Inflammation. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-460561
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460561