The Δ33-35 Mutant α-Domain Containing β-Domain-Like M3S9 Cluster Exhibits the Function of α-Domain with M4S11 Cluster in Human Growth Inhibitory Factor
Joint Authors
Ding, Zhichun
Bao, Qingui
Huang, Zhong-Xian
Tan, Xiangshi
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Neuronal growth inhibitory factor (GIF), also known as metallothionein (metallothionein-3), impairs the survival and neurite formation of cultured neurons.
It is known that the α-β domain-domain interaction of hGIF is crucial to the neuron growth inhibitory bioactivity although the exact mechanism is not clear.
Herein, the β(MT3)-β(MT3) mutant and the hGIF-truncated Δ33-35 mutant were constructed, and their biochemical properties were characterized by pH titration, EDTA, and DTNB reactions.
Their inhibitory activity toward neuron survival and neurite extension was also examined.
We found that the Δ33-35 mutant α-domain containing β-domain-like M3S9 cluster exhibits the function of α-domain with M4S11 cluster in hGIF.
These results showed that the stability and solvent accessibility of the metal-thiolate cluster in β-domain is very significant to the neuronal growth inhibitory activity of hGIF and also indicated that the particular primary structure of α-domain is pivotal to domain-domain interaction in hGIF.
American Psychological Association (APA)
Bao, Qingui& Ding, Zhichun& Huang, Zhong-Xian& Tan, Xiangshi. 2010. The Δ33-35 Mutant α-Domain Containing β-Domain-Like M3S9 Cluster Exhibits the Function of α-Domain with M4S11 Cluster in Human Growth Inhibitory Factor. Bioinorganic Chemistry and Applications،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-461114
Modern Language Association (MLA)
Bao, Qingui…[et al.]. The Δ33-35 Mutant α-Domain Containing β-Domain-Like M3S9 Cluster Exhibits the Function of α-Domain with M4S11 Cluster in Human Growth Inhibitory Factor. Bioinorganic Chemistry and Applications No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-461114
American Medical Association (AMA)
Bao, Qingui& Ding, Zhichun& Huang, Zhong-Xian& Tan, Xiangshi. The Δ33-35 Mutant α-Domain Containing β-Domain-Like M3S9 Cluster Exhibits the Function of α-Domain with M4S11 Cluster in Human Growth Inhibitory Factor. Bioinorganic Chemistry and Applications. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-461114
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461114