Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis
Joint Authors
Wang, Shiyao
Li, Ru
Shi, Jinxia
Sun, Xiaolin
Liu, Yanying
Li, Yanmei
Zhou, Bin
Yu, Ping
Li, Zhanguo
Cui, Liufu
Zhang, Xiaoping
Long, Li
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
This study was to screen for the miRNAs differently expressed in peripheral blood mononuclear cells (PBMC) of RA, to further identify the expression of miR-155 in RA PBMC and fibroblast-like synoviocytes (FLS), and to evaluate the function of miR-155 in RA-FLS.
Methods.
Microarray was used to screen for differentially expressed miRNAs in RA PBMC.
miR-155 expression in PBMC and FLS of RA were identified by real-time PCR.
Enforced overexpression and downexpression of miR-155 were used to investigate the function of miR-155 in RA-FLS.
Expression of IKBKE which was previously identified as the actual target of miR-155 was examined by Western blot and real-time PCR in RA-FLS.
Results.
miR-155 levels were increased in both PBMC and FLS of RA and could be induced by TNF-α.
Upregulation of miR-155 decreased MMP-3 levels and suppressed proliferation and invasion of RA-FLS.
Inverse relationship between the expressions of miR-155 and the MMPs production-related protein IKBKE was found.
Conclusion.
An inflammatory milieu may alter miRNA expression profiles in rheumatoid arthritis.
miR-155 is upregulated in RA-FLS, and it may be a protective factor against the inflammatory effect in part by attenuating expression of IKBKE.
American Psychological Association (APA)
Long, Li& Yu, Ping& Liu, Yanying& Wang, Shiyao& Li, Ru& Shi, Jinxia…[et al.]. 2013. Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-461293
Modern Language Association (MLA)
Long, Li…[et al.]. Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis. Clinical and Developmental Immunology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-461293
American Medical Association (AMA)
Long, Li& Yu, Ping& Liu, Yanying& Wang, Shiyao& Li, Ru& Shi, Jinxia…[et al.]. Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-461293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461293