Noninvasive and Quantitative Assessment of In Vivo Fetomaternal Interface Angiogenesis Using RGD-Based Fluorescence
Joint Authors
Coll, Jean-Luc
Hoffmann, Pascale
Alfaidy, Nadia
Keramidas, M.
Sergent, F.
Brouillet, Sophie
Feige, Jean-Jacques
Lavaud, J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Angiogenesis is a key process for proper placental development and for the success of pregnancy.
Although numerous in vitro methods have been developed for the assessment of this process, relatively few reliable in vivo methods are available to evaluate this activity throughout gestation.
Here we report an in vivo technique that specifically measures placental neovascularization.
The technique is based on the measurement of a fluorescent alpha v beta 3 (αvβ3) integrin-targeting molecule called Angiolone-Alexa-Fluor 700.
The αvβ3 integrin is highly expressed by endothelial cells during the neovascularization and by trophoblast cells during their invasion of the maternal decidua.
Angiolone was injected to gravid mice at 6.5 and 11.5 days post coitus (dpc).
The fluorescence was analyzed one day later at 7.5 and 12.5 dpc, respectively.
We demonstrated that (i) Angiolone targets αvβ3 protein in the placenta with a strong specificity, (ii) this technique is quantitative as the measurement was correlated to the increase of the placental size observed with increasing gestational age, and (iii) information on the outcome is possible, as abnormal placentation could be detected early on during gestation.
In conclusion, we report the validation of a new noninvasive and quantitative method to assess the placental angiogenic activity, in vivo.
American Psychological Association (APA)
Keramidas, M.& Lavaud, J.& Sergent, F.& Hoffmann, Pascale& Brouillet, Sophie& Feige, Jean-Jacques…[et al.]. 2014. Noninvasive and Quantitative Assessment of In Vivo Fetomaternal Interface Angiogenesis Using RGD-Based Fluorescence. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-462327
Modern Language Association (MLA)
Keramidas, M.…[et al.]. Noninvasive and Quantitative Assessment of In Vivo Fetomaternal Interface Angiogenesis Using RGD-Based Fluorescence. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-462327
American Medical Association (AMA)
Keramidas, M.& Lavaud, J.& Sergent, F.& Hoffmann, Pascale& Brouillet, Sophie& Feige, Jean-Jacques…[et al.]. Noninvasive and Quantitative Assessment of In Vivo Fetomaternal Interface Angiogenesis Using RGD-Based Fluorescence. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-462327
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462327