PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats
Joint Authors
Yao, Dawei
Li, Jun
Zhang, Liping
Zhu, Jiangjiang
Shi, Hengbo
Lin, Xianzi
Luo, Jun
Sun, Yuting
Shi, Huaiping
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
To explore the function of PPARγ in the goat mammary gland, we cloned the whole cDNA of the PPARγ gene.
Homology alignments revealed that the goat PPARγ gene is conserved among goat, bovine, mouse, and human.
Luciferase assays revealed that rosiglitazone enhanced the activity of the PPARγ response element (PPRE) in goat mammary epithelial cells (GMECs).
After rosiglitazone (ROSI) treatment of GMECs, there was a significant (P<0.05) increase in the expression of genes related to triacylglycerol synthesis and secretion: LPL, FASN, ACACA, PLIN3, FABP3, PLIN2, PNPLA2, NR1H3, SREBF1, and SCD.
The decreases in expression observed after knockdown of PPARγ relative to the control group (Ad-NC) averaged 65%, 52%, 67%, 55%, 65%, 58%, 85%, 43%, 50%, and 24% for SCD, DGAT1, AGPAT6, SREBF1, ACACA, FASN, FABP3, SCAP, ATGL, and PLIN3, respectively.
These results provide direct evidence that PPARγ plays a crucial role in regulating the triacylglycerol synthesis and secretion in goat mammary cells and underscore the functional importance of PPARγ in mammary gland tissue during lactation.
American Psychological Association (APA)
Shi, Hengbo& Luo, Jun& Zhu, Jiangjiang& Li, Jun& Sun, Yuting& Lin, Xianzi…[et al.]. 2013. PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats. PPAR Research،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462509
Modern Language Association (MLA)
Shi, Hengbo…[et al.]. PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats. PPAR Research No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-462509
American Medical Association (AMA)
Shi, Hengbo& Luo, Jun& Zhu, Jiangjiang& Li, Jun& Sun, Yuting& Lin, Xianzi…[et al.]. PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats. PPAR Research. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462509