Transcriptional Activity of PGC-1α and NT-PGC-1α Is Differentially Regulated by Twist-1 in Brown Fat Metabolism
Joint Authors
Gettys, Thomas W.
Jun, Hee-Jin
Chang, Ji Suk
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Brown fat expresses two PGC-1α isoforms (PGC-1α and NT-PGC-1α) and both play a central role in the regulation of cellular energy metabolism and adaptive thermogenesis by interacting with a wide range of transcription factors including PPARγ, PPARα, ERRα, and NRF1.
PGC-1α consists of 797 amino acids, whereas alternative splicing of the PGC-1α gene produces a shorter protein called NT-PGC-1α (aa 1–270).
We report in this paper that transcriptional activity of PGC-1α and NT-PGC-1α is differently affected by the transcriptional regulator, Twist-1.
Twist-1 suppresses PGC-1α but not NT-PGC-1α.
The inhibition of PGC-1α activity by Twist-1 is mediated by direct interaction through the C-terminal region of PGC-1α (aa 353–797).
Thus, the absence of the corresponding C-terminal domain in NT-PGC-1α allows NT-PGC-1α to be free from Twist-1-mediated inhibition.
Overexpression of Twist-1 in brown adipocytes suppresses transcription of a subset of PGC-1α-target genes involved in mitochondrial fatty acid oxidation and uncoupling (CPT1β, UCP1, and ERRα).
In contrast, NT-PGC-1α-mediated induction of these genes is unaffected by Twist-1.
These findings show that differences in inhibitory protein-protein interactions of PGC-1α and NT-PGC-1α with Twist-1 lead to differential regulation of their function by Twist-1.
American Psychological Association (APA)
Jun, Hee-Jin& Gettys, Thomas W.& Chang, Ji Suk. 2012. Transcriptional Activity of PGC-1α and NT-PGC-1α Is Differentially Regulated by Twist-1 in Brown Fat Metabolism. PPAR Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-463263
Modern Language Association (MLA)
Jun, Hee-Jin…[et al.]. Transcriptional Activity of PGC-1α and NT-PGC-1α Is Differentially Regulated by Twist-1 in Brown Fat Metabolism. PPAR Research No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-463263
American Medical Association (AMA)
Jun, Hee-Jin& Gettys, Thomas W.& Chang, Ji Suk. Transcriptional Activity of PGC-1α and NT-PGC-1α Is Differentially Regulated by Twist-1 in Brown Fat Metabolism. PPAR Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-463263
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463263