p21WAF1CIP1 Expression is Differentially Regulated by Metformin and Rapamycin
Joint Authors
Millward, Ann B.
Tse, Wai
Demaine, Andrew G.
Molnar, Zoltan
Source
International Journal of Chronic Diseases
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-25
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The mammalian target of rapamycin (mTOR) pathway plays an important role in the development of diabetic nephropathy and other age-related diseases.
One of the features of DN is the elevated expression of p21WAF1/CIP1.
However, the importance of the mTOR signalling pathway in p21 regulation is poorly understood.
Here we investigated the effect of metformin and rapamycin on mTOR-related phenotypes in cell lines of epithelial origin.
This study reports that metformin inhibits high glucose-induced p21 expression.
High glucose opposed metformin in regulating cell size, proliferation, and protein synthesis.
These effects were associated with reduced AMPK activation, affecting downstream mTOR signalling.
However, the inhibition of the mTOR pathway by rapamycin did not have a negative effect on p21 expression, suggesting that metformin regulates p21 upstream of mTOR.
These findings provide support for the hypothesis that AMPK activation may regulate p21 expression, which may have implications for diabetic nephropathy and other age-related pathologies.
American Psychological Association (APA)
Molnar, Zoltan& Millward, Ann B.& Tse, Wai& Demaine, Andrew G.. 2014. p21WAF1CIP1 Expression is Differentially Regulated by Metformin and Rapamycin. International Journal of Chronic Diseases،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-463865
Modern Language Association (MLA)
Molnar, Zoltan…[et al.]. p21WAF1CIP1 Expression is Differentially Regulated by Metformin and Rapamycin. International Journal of Chronic Diseases No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-463865
American Medical Association (AMA)
Molnar, Zoltan& Millward, Ann B.& Tse, Wai& Demaine, Andrew G.. p21WAF1CIP1 Expression is Differentially Regulated by Metformin and Rapamycin. International Journal of Chronic Diseases. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-463865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463865