Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides : Detection of Inflammation in a Mouse Model of Hepatitis
Joint Authors
Sclavons, Coralie
Burtea, Carmen
Laurent, Sophie
Muller, Robert N.
Boutry, Sébastien
Vander Elst, Luce
Source
International Journal of Peptides
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
TNF-α is one of the most abundant cytokines produced in many inflammatory and autoimmune conditions such as multiple sclerosis, chronic hepatitis C, or neurodegenerative diseases.
These pathologies remain difficult to diagnose and consequently difficult to treat.
The aim of this work is to offer a new diagnostic tool by seeking new molecular probes for medical imaging.
The target-specific part of the probe consists here of heptameric peptides selected by the phage display technology for their affinity for TNF-α.
Several affinity tests allowed isolating 2 peptides that showed the best binding capacity to TNF-α.
Finally, the best peptide was synthesized in both linear and cyclic forms and tested on the histological sections of concanavalin-A-(ConA-)treated mice liver.
In this well-known hepatitis mouse model, the best results were obtained with the cyclic form of peptide 2, which allowed for the staining of inflamed areas in the liver.
The cyclic form of peptide 2 (2C) was, thus, covalently linked to iron oxide nanoparticles (magnetic resonance imaging (MRI) contrast agent) and tested in the ConA-induced hepatitis mouse model.
The vectorized nanoparticles allowed for the detection of inflammation as well as of the free peptide.
These ex vivo results suggest that phage display-selected peptides can direct imaging contrast agents to inflammatory areas.
American Psychological Association (APA)
Sclavons, Coralie& Burtea, Carmen& Boutry, Sébastien& Laurent, Sophie& Vander Elst, Luce& Muller, Robert N.. 2013. Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides : Detection of Inflammation in a Mouse Model of Hepatitis. International Journal of Peptides،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464732
Modern Language Association (MLA)
Sclavons, Coralie…[et al.]. Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides : Detection of Inflammation in a Mouse Model of Hepatitis. International Journal of Peptides No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-464732
American Medical Association (AMA)
Sclavons, Coralie& Burtea, Carmen& Boutry, Sébastien& Laurent, Sophie& Vander Elst, Luce& Muller, Robert N.. Phage Display Screening for Tumor Necrosis Factor-α-Binding Peptides : Detection of Inflammation in a Mouse Model of Hepatitis. International Journal of Peptides. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464732