In Vitro Antioxidative Evaluation of α- and β-Carotene, Isolated from Crude Palm Oil
Joint Authors
Sen Gupta, Surashree
Ghosh, Mahua
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present work describes the isolation of α- and β-carotene from crude palm oil and their antioxidant potential in an in vitro model.
Pure product was isolated by the method adopted.
Antioxidant activities of the isolated α- and β-carotene were analyzed in five different concentrations of 0.001, 0.005, 0.01, 0.05, and 0.1% (w/v).
From the several assays conducted, an observation was made that the antioxidant activity of the product shifted between antioxidant and prooxidant effects depending on the concentration and the system analyzed.
The metal chelation, DPPH radical scavenging, and superoxide scavenging activities showed almost similar results in terms of high activity at lowest concentrations.
ABTS-scavenging activity was displayed only by a particular antioxidant concentration of 0.1%.
Lipid peroxidation assay pronounced the activity of 0.1% antioxidant in inhibiting oxidation of sensitive bioactive lipids.
In vitro antidenaturation test again specified the efficacy of low concentrations in preventing protein denaturation.
Through this study a definite dosage formulation for consumption of carotenoids is being proposed which will enhance health promotion and prevent chronic diseases when taken as fortified foods or dietary supplements.
American Psychological Association (APA)
Sen Gupta, Surashree& Ghosh, Mahua. 2013. In Vitro Antioxidative Evaluation of α- and β-Carotene, Isolated from Crude Palm Oil. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465043
Modern Language Association (MLA)
Sen Gupta, Surashree& Ghosh, Mahua. In Vitro Antioxidative Evaluation of α- and β-Carotene, Isolated from Crude Palm Oil. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-465043
American Medical Association (AMA)
Sen Gupta, Surashree& Ghosh, Mahua. In Vitro Antioxidative Evaluation of α- and β-Carotene, Isolated from Crude Palm Oil. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465043