Functional Epigenetic Analysis of Prostate Carcinoma : A Role for Seryl-tRNA Synthetase?
Joint Authors
Ikromov, Odiljon
Krause, Hans
Magheli, Ahmed
Alkamal, Imad
Ratert, Nadine
Miller, Kurt
Kempkensteffen, Carsten
Sendeski, Mauricio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Transcriptional silencing, as a result of aberrant promoter hypermethylation, is a common mechanism through which genes in cancer cells become inactive.
Functional epigenetic screens using demethylating agents to reexpress transcriptional silenced genes may identify such inactivated genes for needing further evaluation.
We aimed to identify genes so far not known to be inactivated by promoter hypermethylation in prostate cancer.
DU-145 and LNCaP cells were treated with the DNMT inhibitor zebularine.
Expression changes of total RNA from treated and untreated cells were compared using an RNA expression microarray.
Genes upregulated more than 2-fold were evaluated by RT-qPCR in 50 cases of paired normal and tumor tissues of prostate cancer patients.
SARS was found to be downregulated in prostate cancer in 42/50 cases (84%).
In addition, GADD45A and SPRY4 showed a remarkable diminished expression (88% and 74%, resp.).
The gold standard for promoter hypermethylation-inactivated genes in prostate cancer (GSTP1) was repressed in 90% of our patient samples.
ROC analyses reported statistically significant AUC curves in SARS, GADD45A, and GSTP1 and positive Spearman correlations were found between these genes.
SARS was discovered to be a novel gene that is repressed in prostate cancer and could therefore be recommended for its involvement in prostate carcinogenesis.
American Psychological Association (APA)
Ikromov, Odiljon& Alkamal, Imad& Magheli, Ahmed& Ratert, Nadine& Sendeski, Mauricio& Miller, Kurt…[et al.]. 2014. Functional Epigenetic Analysis of Prostate Carcinoma : A Role for Seryl-tRNA Synthetase?. Journal of Biomarkers،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-465969
Modern Language Association (MLA)
Ikromov, Odiljon…[et al.]. Functional Epigenetic Analysis of Prostate Carcinoma : A Role for Seryl-tRNA Synthetase?. Journal of Biomarkers No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-465969
American Medical Association (AMA)
Ikromov, Odiljon& Alkamal, Imad& Magheli, Ahmed& Ratert, Nadine& Sendeski, Mauricio& Miller, Kurt…[et al.]. Functional Epigenetic Analysis of Prostate Carcinoma : A Role for Seryl-tRNA Synthetase?. Journal of Biomarkers. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-465969
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465969