Teratogenic Effects of Crude Ethanolic Root Bark and Leaf Extracts of Rauwolfia vomitoria (Apocynaceae)‎ on the Femur of Albino Wistar Rat Fetuses

Abstract EN

Introduction.

Rauwolfia vomitoria is a plant used as a sedative and in the treatment of psychotic tendency.

This study was on the teratogenic effects of its root bark and leaf extracts on Wistar rat’s fetal femurs.

Materials and Methods.

Twenty-five female rats weighing between 180 and 200 g were divided into 5 groups, of 5 rats each.

Group A was the control, while Groups B, C, D, and E were the experimental.

The female rats were mated with mature male rats to allow for pregnancy.

Groups B and C animals received orally 150 mg/kg each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, while Groups D and E animals received 250 mg/kg bodyweight each of the root bark and leaf extracts of Rauwolfia vomitoria, respectively, from day 7 to day 11 of gestation.

On day 20 of gestation, the rats were sacrificed, the fetuses were examined, and their femurs were dissected out and preserved, decalcified, and routinely processed using the Haematoxylin and Eosin staining method.

Results.

Histological observations of the fetal femur bones showed numerous osteoblast and osteoclast, hypertrophy, and hyperplasia of bone cells compared with the control.

Conclusion.

Ethanolic root bark and leaf extracts of Rauwolfia vomitoria may lead to advanced skeletal development.