α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5
Joint Authors
Goodison, Steve
Silletti, Steve
Lin, Grace
Lee, Chia-Yao
Marzan, David
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Previous reports demonstrate that the α2-integrin (α2) mediates pancreatic ductal adenocarcinoma (PDAC) cell interactions with collagens.
We found that while well-differentiated cells use α2 exclusively to adhere and migrate on collagenI, poorly differentiated PDAC cells demonstrate reduced reliance on, or complete loss of, α2.
Since well-differentiated PDAC lines exhibit reduced in vitro invasion and α2-blockade suppressed invasion of well-differentiated lines exclusively, we hypothesized that α2 may suppress the malignant phenotype in PDAC.
Accordingly, ectopic expression of α2 retarded in vitro invasion and maintenance on collagenI exacerbated this effect.
Affymetrix profiling revealed that kallikrein-related peptidase-5 (KLK5) was specifically upregulated by α2, and reduced α2 and KLK5 expression was observed in poorly differentiated PDAC cells in situ.
Accordingly, well-differentiated PDAC lines express KLK5, and KLK5 blockade increased the invasion of KLK5-positive lines.
The α2-cytoplasmic domain was dispensable for these effects, demonstrating that the α2-ectodomain and KLK5 coordinately regulate a less invasive phenotype in PDAC.
American Psychological Association (APA)
Lee, Chia-Yao& Marzan, David& Lin, Grace& Goodison, Steve& Silletti, Steve. 2011. α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5. Journal of Oncology،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-466274
Modern Language Association (MLA)
Lee, Chia-Yao…[et al.]. α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5. Journal of Oncology No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-466274
American Medical Association (AMA)
Lee, Chia-Yao& Marzan, David& Lin, Grace& Goodison, Steve& Silletti, Steve. α2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5. Journal of Oncology. 2011. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-466274
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466274