Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
Joint Authors
Balslev, Eva
Kofoed, Vibeke
Dabetic, Tanja
Espersen, Maiken L. M.
Høgdall, Estrid
Poulsen, Tim S.
Source
International Journal of Breast Cancer
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The purpose was to evaluate and compare 5 different HER2 genetic assays with different characteristics that could affect the performance to analyze the human epidermal growth factor 2 (HER2) gene copy number under low and high throughput conditions.
The study included 108 tissue samples from breast cancer patients with HER2 immunohistochemistry (IHC) results scored as 0/1+, 2+, and 3+.
HER2 genetic status was analysed using chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH).
Scoring results were documented through digital image analysis.
The cancer region of interest was identified from a serial H&E stained slide following tissue cores were transferred to a tissue microarrays (TMA).
When using TMA in a routine flow, all patients will be tested for HER2 status with IHC followed by CISH or FISH, thereby providing individual HER2 results.
In conclusion, our results show that the differences between the HER2 genetic assays do not have an effect on the analytic performance and the CISH technology is superior to high throughput HER2 genetic testing due to scanning speed, while the IQ-FISH may still be a choice for fast low throughput HER2 genetic testing.
American Psychological Association (APA)
Poulsen, Tim S.& Espersen, Maiken L. M.& Kofoed, Vibeke& Dabetic, Tanja& Høgdall, Estrid& Balslev, Eva. 2013. Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing. International Journal of Breast Cancer،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-466495
Modern Language Association (MLA)
Poulsen, Tim S.…[et al.]. Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing. International Journal of Breast Cancer No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-466495
American Medical Association (AMA)
Poulsen, Tim S.& Espersen, Maiken L. M.& Kofoed, Vibeke& Dabetic, Tanja& Høgdall, Estrid& Balslev, Eva. Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing. International Journal of Breast Cancer. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-466495
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466495