Bioprocessing of “Hair Waste” by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive : Characterization and Wash Performance Analysis
Joint Authors
Cavalitto, Sebastián F.
Hours, Roque A.
Cavello, Ivana A.
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Information Technology and Computer Science
Abstract EN
Paecilomyces lilacinus (Thom) Samson LPS 876, a locally isolated fungal strain, was grown on minimal mineral medium containing “hair waste,” a residue from the hair-saving unhairing process, and produced a protease with keratinolytic activity.
This enzyme was biochemically characterized.
The optimum reaction conditions, determined with a response surface methodology, were 60°C and pH 6.0.
It was remarkably stable in a wide range of pHs and temperatures.
Addition of Ca2+, Mg2+, or sorbitol was found to be effective in increasing thermal stability of the protease.
PMSF and Hg2+ inhibited the proteolytic activity indicating the presence of a thiol-dependent serine protease.
It showed high stability toward surfactants, bleaching agents, and solvents.
It was also compatible with commercial detergents (7 mg/mL) such as Ariel, Skip, Drive, and Ace, retaining more than 70% of its proteolytic activity in all detergents after 1 h of incubation at 40°C.
Wash performance analysis revealed that this protease could effectively remove blood stains.
From these properties, this enzyme may be considered as a potential candidate for future use in biotechnological processes, as well as in the formulation of laundry detergents.
American Psychological Association (APA)
Cavello, Ivana A.& Hours, Roque A.& Cavalitto, Sebastián F.. 2012. Bioprocessing of “Hair Waste” by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive : Characterization and Wash Performance Analysis. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-466541
Modern Language Association (MLA)
Cavello, Ivana A.…[et al.]. Bioprocessing of “Hair Waste” by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive : Characterization and Wash Performance Analysis. Biotechnology Research International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-466541
American Medical Association (AMA)
Cavello, Ivana A.& Hours, Roque A.& Cavalitto, Sebastián F.. Bioprocessing of “Hair Waste” by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive : Characterization and Wash Performance Analysis. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-466541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466541