Contribution of Alanine-76 and Serine Phosphorylation in α-Synuclein Membrane Association and Aggregation in Yeasts
Joint Authors
Herrera, Sara
Solvang, Keith
Brezinsky, Rebecca
White, Michael
DebBurman, Shubhik
Valtierra, Stephanie
Zorniak, Michael
Fiske, Michael
Konnikova, Alina
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In Parkinson's disease (PD), misfolded and aggregated α-synuclein protein accumulates in degenerating midbrain dopaminergic neurons.
The amino acid alanine-76 in α-synuclein and phosphorylation at serine-87 and serine-129 are thought to regulate its aggregation and toxicity.
However, their exact contributions to α-synuclein membrane association are less clear.
We found that α-synuclein is indeed phosphorylated in fission yeast and budding yeast, the two models that we employed for assessing α-synuclein aggregation and membrane association properties, respectively.
Surprisingly, blocking serine phosphorylation (S87A, S129A, and S87A/S129A) or mimicking it (S87D, S129D) altered α-synuclein aggregation in fission yeast.
Either blocking or mimicking this phosphorylation increased endomembrane association in fission yeast, but only mimicking it decreased plasma membrane association in budding yeast.
Polar substitution mutations of alanine-76 (A76E and A76R) decreased α-synuclein membrane association in budding yeast and decreased aggregation in fission yeast.
These yeast studies extend our understanding of serine phosphorylation and alanine-76 contributions to α-synuclein aggregation and are the first to detail their impact on α-synuclein's plasma membrane and endomembrane association.
American Psychological Association (APA)
Fiske, Michael& Valtierra, Stephanie& Solvang, Keith& Zorniak, Michael& White, Michael& Herrera, Sara…[et al.]. 2011. Contribution of Alanine-76 and Serine Phosphorylation in α-Synuclein Membrane Association and Aggregation in Yeasts. Abstract and Applied Analysis،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-468516
Modern Language Association (MLA)
Fiske, Michael…[et al.]. Contribution of Alanine-76 and Serine Phosphorylation in α-Synuclein Membrane Association and Aggregation in Yeasts. Abstract and Applied Analysis No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-468516
American Medical Association (AMA)
Fiske, Michael& Valtierra, Stephanie& Solvang, Keith& Zorniak, Michael& White, Michael& Herrera, Sara…[et al.]. Contribution of Alanine-76 and Serine Phosphorylation in α-Synuclein Membrane Association and Aggregation in Yeasts. Abstract and Applied Analysis. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-468516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468516