Interleukin-1α Induction in Human Keratinocytes (HaCaT) : An In Vitro Model for Chemoprevention in Skin
Joint Authors
Magcwebeba, T.
Riedel, S.
Gelderblom, W.
Swart, P.
Bouic, P.
Swanevelder, S.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Long-term exposure to UV irradiation and toxic chemicals is associated with chronic inflammation that contributes to skin cancer development with interleukin-1 alpha (IL-1α), constitutively produced by keratinocytes, playing a pivotal role in skin inflammation.
The aim of this study was to investigate the modulation of IL-1α production in the HaCaT keratinocyte cell line.
Phorbol 12-myristate 13-acetate failed to induce IL-1α in HaCaT cells, and this might be associated with the specific deficiency known to affect downstream signalling of the MEK/ERK pathway in these cells.
The calcium ionophore, ionomycin, slightly enhanced the production of intracellular (icIL-1α), but this resulted in a necrotic release at higher concentrations.
UV-B exposure significantly increased the production of icIL-1α in a dose-dependent manner with a maximal induction exhibited at 24 h with minimal necrotic and apoptotic effects.
Validation of the HaCaT cell model indicated that the nonsteroidal anti-inflammatory drug (NSAID), ibuprofen, and the glucocorticoid, dexamethasone, inhibited icIL-1α production, and this was associated with a slight inhibition of cell viability.
The UV-B-induced keratinocyte cell model provides an in vitro system that could, apart from phorbol ester-like compounds, be utilised as a screening assay in identifying skin irritants and/or therapeutic topical agents via the modulation of IL-1α production.
American Psychological Association (APA)
Magcwebeba, T.& Riedel, S.& Swanevelder, S.& Bouic, P.& Swart, P.& Gelderblom, W.. 2012. Interleukin-1α Induction in Human Keratinocytes (HaCaT) : An In Vitro Model for Chemoprevention in Skin. Journal of Skin Cancer،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468621
Modern Language Association (MLA)
Magcwebeba, T.…[et al.]. Interleukin-1α Induction in Human Keratinocytes (HaCaT) : An In Vitro Model for Chemoprevention in Skin. Journal of Skin Cancer No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-468621
American Medical Association (AMA)
Magcwebeba, T.& Riedel, S.& Swanevelder, S.& Bouic, P.& Swart, P.& Gelderblom, W.. Interleukin-1α Induction in Human Keratinocytes (HaCaT) : An In Vitro Model for Chemoprevention in Skin. Journal of Skin Cancer. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-468621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468621