Skip Regulates TGF-β1-Induced Extracellular Matrix Degrading Proteases Expression in Human PC-3 Prostate Cancer Cells
Joint Authors
Villar, Victor
Kocić, Jelena
Santibanez, Juan F.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
To determine whether Ski-interacting protein (SKIP) regulates TGF-β1-stimulated expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9), and uPA Inhibitor (PAI-1) in the androgen-independent human prostate cancer cell model.
Materials and Methods.
PC-3 prostate cancer cell line was used.
The role of SKIP was evaluated using synthetic small interference RNA (siRNA) compounds.
The expression of uPA, MMP-9, and PAI-1 was evaluated by zymography assays, RT-PCR, and promoter transactivation analysis.
Results.
In PC-3 cells TGF-β1 treatment stimulated uPA, PAI-1, and MMP-9 expressions.
The knockdown of SKIP in PC-3 cells enhanced the basal level of uPA, and TGF-β1 treatment inhibited uPA production.
Both PAI-1 and MMP-9 production levels were increased in response to TGF-β1.
The ectopic expression of SKIP inhibited both TGF-β1-induced uPA and MMP-9 promoter transactivation, while PAI-1 promoter response to the factor was unaffected.
Conclusions.
SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-β1 in PC-3 cells.
Thus, SKIP is implicated in the regulation of extracellular matrix degradation and can therefore be suggested as a novel therapeutic target in prostate cancer treatment.
American Psychological Association (APA)
Villar, Victor& Kocić, Jelena& Santibanez, Juan F.. 2013. Skip Regulates TGF-β1-Induced Extracellular Matrix Degrading Proteases Expression in Human PC-3 Prostate Cancer Cells. Prostate Cancer،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-468997
Modern Language Association (MLA)
Villar, Victor…[et al.]. Skip Regulates TGF-β1-Induced Extracellular Matrix Degrading Proteases Expression in Human PC-3 Prostate Cancer Cells. Prostate Cancer No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-468997
American Medical Association (AMA)
Villar, Victor& Kocić, Jelena& Santibanez, Juan F.. Skip Regulates TGF-β1-Induced Extracellular Matrix Degrading Proteases Expression in Human PC-3 Prostate Cancer Cells. Prostate Cancer. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-468997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-468997