Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization
Joint Authors
Behlke, Mark A.
Wengel, Jesper
Langel, Ülo
Muñoz-Alarcón, Andrés
EL Andaloussi, Samir
Romero, Cristian
Lennox, Kim A.
Guterstam, Peter
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Pharmacy, Health & Medical Sciences
Pharmacology
Abstract EN
MicroRNAs are short, endogenous RNAs that direct posttranscriptional regulation of gene expression vital for many developmental and cellular functions.
Implicated in the pathogenesis of several human diseases, this group of RNAs provides interesting targets for therapeutic intervention.
Anti-microRNA oligonucleotides constitute a class of synthetic antisense oligonucleotides used to interfere with microRNAs.
In this study, we investigate the effects of chemical modifications and truncations on activity and specificity of anti-microRNA oligonucleotides targeting microRNA-21.
We observed an increased activity but reduced specificity when incorporating locked nucleic acid monomers, whereas the opposite was observed when introducing unlocked nucleic acid monomers.
Our data suggest that phosphorothioate anti-microRNA oligonucleotides yield a greater activity than their phosphodiester counterparts and that a moderate truncation of the anti-microRNA oligonucleotide improves specificity without significantly losing activity.
These results provide useful insights for design of anti-microRNA oligonucleotides to achieve both high activity as well as efficient mismatch discrimination.
American Psychological Association (APA)
Muñoz-Alarcón, Andrés& Guterstam, Peter& Romero, Cristian& Behlke, Mark A.& Lennox, Kim A.& Wengel, Jesper…[et al.]. 2012. Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization. ISRN Pharmaceutics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-469656
Modern Language Association (MLA)
Muñoz-Alarcón, Andrés…[et al.]. Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization. ISRN Pharmaceutics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-469656
American Medical Association (AMA)
Muñoz-Alarcón, Andrés& Guterstam, Peter& Romero, Cristian& Behlke, Mark A.& Lennox, Kim A.& Wengel, Jesper…[et al.]. Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization. ISRN Pharmaceutics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-469656
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469656