Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens
Joint Authors
Khodadadi, Ali
Borsi, Seyed Hamid
Amini, Akram
Shamsbiranvand, Mohammad-Hosein
Assarehzadegan, Mohammad-Ali
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Pollen from the Acacia has been reported as an important source of pollinosis in tropical and subtropical regions of the world.
The aim of this study was to characterize the IgE binding protein of Acacia farnesiana pollen extract and evaluate cross-reactivity with the most allergenic pollens.
In this study, pollen extract was fractionated by SDS-PAGE and the allergenic profile was determined by IgE-immunoblotting and specific ELISA using forty-two Acacia allergic patients.
Potential cross-reactivity among Acacia and selected allergenic plants was evaluated with ELISA and immunoblotting inhibition experiments.
There were several resolved protein fractions on SDS-PAGE which ranged from 12 to 85 kDa.
Several allergenic protein bands with molecular weights approximately between 12 and 85 kDa were recognized by IgE-specific antibodies from Acacia allergic patients in the immunoblot assay.
The inhibition by the Prosopis juliflora pollen extract was more than those by other pollen extracts.
Moreover, the wheal diameters generated by the Acacia pollen extract were highly correlated with those of P.
juliflora pollen extracts.
The findings suggest that several proteins such as 15, 23, 45, and 50 kDa proteins could be used as diagnostic and therapeutic reagents for patients allergic to A.
farnesiana and P.
juliflora.
American Psychological Association (APA)
Shamsbiranvand, Mohammad-Hosein& Khodadadi, Ali& Assarehzadegan, Mohammad-Ali& Borsi, Seyed Hamid& Amini, Akram. 2014. Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens. Journal of Allergy،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469843
Modern Language Association (MLA)
Shamsbiranvand, Mohammad-Hosein…[et al.]. Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens. Journal of Allergy No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-469843
American Medical Association (AMA)
Shamsbiranvand, Mohammad-Hosein& Khodadadi, Ali& Assarehzadegan, Mohammad-Ali& Borsi, Seyed Hamid& Amini, Akram. Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens. Journal of Allergy. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-469843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-469843