Gamma-Tocotrienol Modulated Gene Expression in Senescent Human Diploid Fibroblasts as Revealed by Microarray Analysis
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Zainuddin, Azalina
Kien Hui, Chua
Ngah, Wan Zurinah Wan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The effect of γ-tocotrienol, a vitamin E isomer, in modulating gene expression in cellular aging of human diploid fibroblasts was studied.
Senescent cells at passage 30 were incubated with 70 μM of γ-tocotrienol for 24 h.
Gene expression patterns were evaluated using Sentrix HumanRef-8 Expression BeadChip from Illumina, analysed using GeneSpring GX10 software, and validated using quantitative RT-PCR.
A total of 100 genes were differentially expressed (P<0.001) by at least 1.5 fold in response to γ-tocotrienol treatment.
Amongst the genes were IRAK3, SelS, HSPA5, HERPUD1, DNAJB9, SEPR1, C18orf55, ARF4, RINT1, NXT1, CADPS2, COG6, and GLRX5.
Significant gene list was further analysed by Gene Set Enrichment Analysis (GSEA), and the Normalized Enrichment Score (NES) showed that biological processes such as inflammation, protein transport, apoptosis, and cell redox homeostasis were modulated in senescent fibroblasts treated with γ-tocotrienol.
These findings revealed that γ-tocotrienol may prevent cellular aging of human diploid fibroblasts by modulating gene expression.
American Psychological Association (APA)
Makpol, Suzana& Zainuddin, Azalina& Kien Hui, Chua& Mohd Yusof, Yasmin Anum& Ngah, Wan Zurinah Wan. 2013. Gamma-Tocotrienol Modulated Gene Expression in Senescent Human Diploid Fibroblasts as Revealed by Microarray Analysis. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-472864
Modern Language Association (MLA)
Makpol, Suzana…[et al.]. Gamma-Tocotrienol Modulated Gene Expression in Senescent Human Diploid Fibroblasts as Revealed by Microarray Analysis. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-472864
American Medical Association (AMA)
Makpol, Suzana& Zainuddin, Azalina& Kien Hui, Chua& Mohd Yusof, Yasmin Anum& Ngah, Wan Zurinah Wan. Gamma-Tocotrienol Modulated Gene Expression in Senescent Human Diploid Fibroblasts as Revealed by Microarray Analysis. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-472864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-472864
