Effects of β2 Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts
Joint Authors
Oliver, Brian G.
King, Nicholas J.C.
Van Ly, David
Burgess, Janette K.
Moir, Lyn M.
Black, Judith L.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Rhinovirus-(RV-) induced asthma exacerbations account for high asthma-related health costs and morbidity in Australia.
The cellular mechanism underlying this pathology is likely the result of RV-induced nuclear-factor-kappa-B-(NF-κB-) dependent inflammation.
NF-κB may also be important in RV replication as inhibition of NF-κB inhibits replication of other viruses such as human immunodeficiency virus and cytomegalovirus.
To establish the role of NF-κB inhibitors in RV-induced IL- 6 and IL-8 and RV replication, we used pharmacological inhibitors of NF-κB, and steroids and/or β2 agonists were used for comparison.
Primary human lung fibroblasts were infected with RV-16 in the presence of NF-κB inhibitors: BAY-117085 and dimethyl fumarate; β2 agonist: salmeterol; and/or corticosteroids: dexamethasone; fluticasone.
RV-induced IL-6 and IL-8 and RV replication were assessed using ELISAs and virus titration assays.
RV replicated and increased IL-6 and IL-8 release.
Salmeterol increased, while dexamethasone and fluticasone decreased RV-induced IL-6 and IL-8 (P<0.05).
The NF-κB inhibitor BAY-117085 inhibited only RV-induced IL-6 (P<0.05) and dimethyl fumarate did not alter RV-induced IL-6 and IL-8.
Dimethylfumarate increased RV replication whilst other drugs did not alter RV replication.
These data suggest that inhibition of NF-κB alone is unlikely to be an effective treatment compared to current asthma therapeutics.
American Psychological Association (APA)
Van Ly, David& King, Nicholas J.C.& Moir, Lyn M.& Burgess, Janette K.& Black, Judith L.& Oliver, Brian G.. 2011. Effects of β2 Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts. Journal of Allergy،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-473018
Modern Language Association (MLA)
Van Ly, David…[et al.]. Effects of β2 Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts. Journal of Allergy No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-473018
American Medical Association (AMA)
Van Ly, David& King, Nicholas J.C.& Moir, Lyn M.& Burgess, Janette K.& Black, Judith L.& Oliver, Brian G.. Effects of β2 Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts. Journal of Allergy. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-473018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473018