Comparative Transcriptome Analysis of Leaves and Roots in Response to Sudden Increase in Salinity in Brassica napus by RNA-seq
Joint Authors
Chow, Kingsley
Kwan, Bih-Hua
Kok, Eng-Piew
Yong, Hui-Yee
Kitashiba, Hiroyasu
Nanzyo, Masami
Nishio, Takeshi
Zou, Zhongwei
Nasu, Shiori
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-07
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Amphidiploid species in the Brassicaceae family, such as Brassica napus, are more tolerant to environmental stress than their diploid ancestors. A relatively salt tolerant B.
napus line, N119, identified in our previous study, was used.
N119 maintained lower Na+ content, and Na+/K+ and Na+/Ca2+ ratios in the leaves than a susceptible line.
The transcriptome profiles of both the leaves and the roots 1 h and 12 h after stress were investigated.
De novo assembly of individual transcriptome followed by sequence clustering yielded 161,537 nonredundant sequences.
A total of 14,719 transcripts were differentially expressed in either organs at either time points.
GO and KO enrichment analyses indicated that the same 49 GO terms and seven KO terms were, respectively, overrepresented in upregulated transcripts in both organs at 1 h after stress.
Certain overrepresented GO term of genes upregulated at 1 h after stress in the leaves became overrepresented in genes downregulated at 12 h.
A total of 582 transcription factors and 438 transporter genes were differentially regulated in both organs in response to salt shock.
The transcriptome depicting gene network in the leaves and the roots regulated by salt shock provides valuable information on salt resistance genes for future application to crop improvement.
American Psychological Association (APA)
Yong, Hui-Yee& Zou, Zhongwei& Kok, Eng-Piew& Kwan, Bih-Hua& Chow, Kingsley& Nasu, Shiori…[et al.]. 2014. Comparative Transcriptome Analysis of Leaves and Roots in Response to Sudden Increase in Salinity in Brassica napus by RNA-seq. BioMed Research International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-473816
Modern Language Association (MLA)
Yong, Hui-Yee…[et al.]. Comparative Transcriptome Analysis of Leaves and Roots in Response to Sudden Increase in Salinity in Brassica napus by RNA-seq. BioMed Research International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-473816
American Medical Association (AMA)
Yong, Hui-Yee& Zou, Zhongwei& Kok, Eng-Piew& Kwan, Bih-Hua& Chow, Kingsley& Nasu, Shiori…[et al.]. Comparative Transcriptome Analysis of Leaves and Roots in Response to Sudden Increase in Salinity in Brassica napus by RNA-seq. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-473816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473816