Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells
Joint Authors
Foerster, Martin
Figulla, Hans Reiner
Wang, Zhenhua
Meusel, Katharina
Franz, Marcus
Jung, Christian
Fritzenwanger, Michael
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chronic heart failure (CHF) is associated with elevated concentrations of tumor necrosis factor (TNF) α and cardiotrophin-1 (CT-1) and altered peripheral blood mononuclear cell (PBMC) function.
Therefore, we tested whether CT-1 induces TNFα in PBMC of healthy volunteers.
CT-1 induced in PBMC TNFα protein in the supernatant and TNFα mRNA in a concentration- and time-dependent manner determined by ELISA and real-time PCR, respectively.
Maximal TNFα protein was achieved with 100 ng/mL CT-1 after 3–6 hours and maximal TNFα mRNA induction after 1 hour.
ELISA data were confirmed using immunofluorescent flow cytometry.
Inhibitor studies with actinomycin D and brefeldin A showed that both protein synthesis and intracellular transport are essential for CT-1 induced TNFα expression.
CT-1 caused a dose dependent nuclear factor (NF) κB translocation.
Parthenolide inhibited both NFκB translocation and TNFα protein expression indicating that NFκB seems to be necessary.
We revealed a new mechanism for elevated serum TNFα concentrations and PBMC activation in CHF besides the hypothesis of PBMC activation by bacterial translocation from the gut.
American Psychological Association (APA)
Fritzenwanger, Michael& Meusel, Katharina& Jung, Christian& Franz, Marcus& Wang, Zhenhua& Foerster, Martin…[et al.]. 2010. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-475710
Modern Language Association (MLA)
Fritzenwanger, Michael…[et al.]. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-475710
American Medical Association (AMA)
Fritzenwanger, Michael& Meusel, Katharina& Jung, Christian& Franz, Marcus& Wang, Zhenhua& Foerster, Martin…[et al.]. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-475710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475710