Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models
Joint Authors
Herrera, Sara
White, Michael
DebBurman, Shubhik
Solvang, Keith
Valtierra, Stephanie
Fiske, Michael
Konnikova, Alina
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In Parkinson’s disease (PD), midbrain dopaminergic neuronal death is linked to the accumulation of aggregated α-synuclein.
The familial PD mutant form of α-synuclein, E46K, has not been thoroughly evaluated yet in an organismal model system.
Here, we report that E46K resembled wild-type (WT) α-synuclein in Saccharomyces cerevisiae in that it predominantly localized to the plasma membrane, and it did not induce significant toxicity or accumulation.
In contrast, in Schizosaccharomyces pombe, E46K did not associate with the plasma membrane.
Instead, in one strain, it extensively aggregated in the cytoplasm and was as toxic as WT.
Remarkably, in another strain, E46K extensively associated with the endomembrane system and was more toxic than WT.
Our studies recapitulate and extend aggregation and phospholipid membrane association properties of E46K previously observed in vitro and cell culture.
Furthermore, it supports the notion that E46K generates toxicity partly due to increased association with endomembrane systems within cells.
American Psychological Association (APA)
Fiske, Michael& White, Michael& Valtierra, Stephanie& Herrera, Sara& Solvang, Keith& Konnikova, Alina…[et al.]. 2011. Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models. ISRN Neurology،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-478382
Modern Language Association (MLA)
Fiske, Michael…[et al.]. Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models. ISRN Neurology No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-478382
American Medical Association (AMA)
Fiske, Michael& White, Michael& Valtierra, Stephanie& Herrera, Sara& Solvang, Keith& Konnikova, Alina…[et al.]. Familial Parkinson's Disease Mutant E46K α-Synuclein Localizes to Membranous Structures, Forms Aggregates, and Induces Toxicity in Yeast Models. ISRN Neurology. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-478382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478382