PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells
Joint Authors
Wang, Xian
Li, Fuwang
Hong, Tian-pei
Lu, Changlin
An, Huijie
Wang, Haining
Wang, Guang
Liu, Jinbo
Hao, Yanting
Chen, Alex F.
He, Liyun
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Tetrahydrobiopterin (BH4) is an essential cofactor for endothelial nitric oxide (NO) synthase.
Guanosine 5′-triphosphate cyclohydrolase-I (GTPCH-I) is a key limiting enzyme for BH4 synthesis.
In the present in vitro study, we investigated whether peroxisome proliferator-activated receptor α (PPAR-α) agonist fenofibrate could recouple eNOS by reversing low-expression of intracellular BH4 in endothelial cells and discussed the potential mechanisms.
After human umbilical vein endothelial cells (HUVECs) were treated with lipopolysaccharide (LPS) for 24 hours, the levels of cellular eNOS, BH4 and cell supernatant NO were significantly reduced compared to control group.
And the fluorescence intensity of intracellular ROS was significantly increased.
But pretreated with fenofibrate (10 umol/L) for 2 hours before cells were induced by LPS, the levels of eNOS, NO, and BH4 were significantly raised compared to LPS treatment alone.
ROS production was markedly reduced in fenofibrate group than LPS group.
In addition, our results showed that the level of intracellular GTPCH-I detected by western blot was increased in a concentration-dependent manner after being treated with fenofibrate.
These results suggested that fenofibrate might help protect endothelial function and against atherosclerosis by increasing level of BH4 and decreasing production of ROS through upregulating the level of intracellular GTPCH-I.
American Psychological Association (APA)
Liu, Jinbo& Lu, Changlin& Li, Fuwang& Wang, Haining& He, Liyun& Hao, Yanting…[et al.]. 2011. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478434
Modern Language Association (MLA)
Liu, Jinbo…[et al.]. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-478434
American Medical Association (AMA)
Liu, Jinbo& Lu, Changlin& Li, Fuwang& Wang, Haining& He, Liyun& Hao, Yanting…[et al.]. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478434
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478434