Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria : Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?
Joint Authors
Mayr, Barbara
Wallner, Franz
Habres, Claudia
Enko, Dietmar
Halwachs-Baumann, Gabriele
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria.
Routine blood samples of 2420 patients were investigated.
The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System.
The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel.
In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result.
Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies.
Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e.
In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test.
The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies.
(Trial Registration: K-37-13).
American Psychological Association (APA)
Enko, Dietmar& Habres, Claudia& Wallner, Franz& Mayr, Barbara& Halwachs-Baumann, Gabriele. 2014. Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria : Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?. Journal of Blood Transfusion،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-479306
Modern Language Association (MLA)
Enko, Dietmar…[et al.]. Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria : Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?. Journal of Blood Transfusion No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-479306
American Medical Association (AMA)
Enko, Dietmar& Habres, Claudia& Wallner, Franz& Mayr, Barbara& Halwachs-Baumann, Gabriele. Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria : Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?. Journal of Blood Transfusion. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-479306
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479306