Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae
Joint Authors
Galleni, Moreno
Mercuri, Paola Sandra
Belhadj, Omrane
Ben Achour, Nahed
Ben Moussa, Mohamed
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Klebsiella pneumoniae ML2011, a multiresistant isolate, was isolated from the Military Hospital of Tunis (Tunisia).
The determination of the minimal inhibitory concentrations exhibited by K.
pneumoniae ML2011 was performed by Etest.
The crude extract of the isolates contains four different β-lactamases with pI 5.5, 7.3, 7.6, and 8.6.
Only the β-lactamases with pI 7.3 and pI 8.6 were transferred by transformation and conjugation experiment.
Molecular characterization of these genes was performed by PCR and sequencing.
The chromosomal β-lactamases are TEM (pI 5.5) and SHV-1 (7.6).
CTX-M-28 (pI 8.6) and the novel variant of SHV named SHV-104 (pI 7.3) were encoded by bla gene located on a 50 kb highly conjugative plasmid.
The SHV-104 β-lactamase was produced in E.
coli and purified.
Its profile of activity was determined.
Compared to SHV-1, SHV-104 contains one mutation, R202S.
Their kinetic parameters were similar except for cefotaxime.
The analysis of the predicted structure of SHV-104 indicated that the R202S mutation suppresses a salt bridge present in SHV-1.
Therefore, the overall flexibility of the protein increased and might improve the hydrolysis of cefotaxime.
We can conclude that the multiresistant phenotype of K.
pneumoniae ML2011 strain is mainly linked to the production of CTX-M-28 since SHV-104 possesses a narrow spectrum of activity.
American Psychological Association (APA)
Ben Achour, Nahed& Belhadj, Omrane& Galleni, Moreno& Ben Moussa, Mohamed& Mercuri, Paola Sandra. 2014. Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-480584
Modern Language Association (MLA)
Ben Achour, Nahed…[et al.]. Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae. International Journal of Microbiology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-480584
American Medical Association (AMA)
Ben Achour, Nahed& Belhadj, Omrane& Galleni, Moreno& Ben Moussa, Mohamed& Mercuri, Paola Sandra. Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-480584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480584