A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1
Joint Authors
Asano, Yasuhisa
Isobe, Kimiyasu
Nagasawa, Shouko
Fuhshuku, Ken-ichi
Tamauchi, Hiroshi
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple enzymatic method for production of a wide variety of D-amino acids was developed by kinetic resolution of DL-amino acids using L-amino acid oxidase (L-AAO) with broad substrate specificity from Rhodococcus sp.
AIU Z-35-1.
The optimum pH of the L-AAO reaction was classified into three groups depending on the L-amino acids as substrate, and their respective activities between pH 5.5 and 8.5 accounted for more than 60% of the optimum activity.
The enzyme was stable in the range from pH 6.0 to 8.0, and approximately 80% of the enzyme activity remained after incubation at 40∘C for 60 min at pH 7.0.
D-Amino acids such as D-citrulline, D-glutamine, D-homoserine or D-arginine, which are not produced by D-aminoacylases or D-hydantoinases, were produced from the racemic mixture within a 24-hr reaction at 30∘C and pH 7.0.
Thus, the present method using L-AAO was versatile for production of a wide variety of D-amino acids.
American Psychological Association (APA)
Isobe, Kimiyasu& Tamauchi, Hiroshi& Fuhshuku, Ken-ichi& Nagasawa, Shouko& Asano, Yasuhisa. 2010. A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1. Enzyme Research،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-481285
Modern Language Association (MLA)
Isobe, Kimiyasu…[et al.]. A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1. Enzyme Research No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-481285
American Medical Association (AMA)
Isobe, Kimiyasu& Tamauchi, Hiroshi& Fuhshuku, Ken-ichi& Nagasawa, Shouko& Asano, Yasuhisa. A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-481285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481285