Intranasal Immunization with ChitosanpCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model
Joint Authors
Yin, Yan-Xin
Pan, Zhi-ming
Jiao, Xin-an
Zhang, Gong
Liu, Xiu-fan
Huang, Jin-lin
Zhu, Ai-ping
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Campylobacter jejuni is the most common zoonotic bacterium associated with human diarrhea, and chickens are considered to be one of the most important sources for human infection, with no effective prophylactic treatment available.
We describe here a prophylactic strategy using chitosan-DNA intranasal immunization to induce specific immune responses.
The chitosan used for intranasal administration is a natural mucus absorption enhancer, which results in transgenic DNA expression in chicken nasopharynx.
Chickens immunized with chitosan-DNA nanoparticles, which carried a gene for the major structural protein FlaA, produced significantly increased levels of serum anti-Campylobacter jejuni IgG and intestinal mucosal antibody (IgA), compared to those treated with chitosan-DNA (pCAGGS).
Chitosan-pCAGGS-flaA intranasal immunization induced reductions of bacterial expellation by 2-3 log10 and 2 log10 in large intestine and cecum of chickens, respectively, when administered with the isolated C.
jejuni strain.
This study demonstrated that intranasal delivery of chitosan-DNA vaccine successfully induced effective immune response and might be a promising vaccine candidate against C.
jejuni infection.
American Psychological Association (APA)
Huang, Jin-lin& Yin, Yan-Xin& Pan, Zhi-ming& Zhang, Gong& Zhu, Ai-ping& Liu, Xiu-fan…[et al.]. 2010. Intranasal Immunization with ChitosanpCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-483152
Modern Language Association (MLA)
Huang, Jin-lin…[et al.]. Intranasal Immunization with ChitosanpCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-483152
American Medical Association (AMA)
Huang, Jin-lin& Yin, Yan-Xin& Pan, Zhi-ming& Zhang, Gong& Zhu, Ai-ping& Liu, Xiu-fan…[et al.]. Intranasal Immunization with ChitosanpCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-483152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483152