Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGEMS
Joint Authors
Polenakovic, Momir
Komina, Selim
Stankov, Oliver
Davalieva, Katarina
Kiprijanovska, Sanja
Stavridis, Sotir
Petrusevska, Gordana
Source
International Journal of Proteomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Proteome analysis of the urine has shown that urine contains disease-specific information for a variety of urogenital system disorders, including prostate cancer (PCa).
The aim of this study was to determine the protein components of urine from PCa patients.
Urine from 8 patients with clinically and histologically confirmed PCa was analyzed by conventional 2D PAGE.
The MS identification of the most prominent 125 spots from the urine map revealed 45 distinct proteins.
According to Gene Ontology, the identified proteins are involved in a variety of biological processes, majority of them are secreted (71%), and half of them are enzymes or transporters.
Comparison with the normal urine proteome revealed 11 proteins distinctive for PCa.
Using Ingenuity Pathways Analysis, we have found 3 proteins (E3 ubiquitin-protein ligase rififylin, tumor protein D52, and thymidine phosphorylase) associated with cellular growth and proliferation (p=8.35×10-4-3.41×10-2).
The top network of functional associations between 11 proteins was Cell Death and Survival, Cell-To-Cell Signaling and Interaction, and System Development and Function (p=10-30).
In summary, we have created an initial proteomic map of PCa patient’s urine.
The results from this study provide some leads to understand the molecular bases of prostate cancer.
American Psychological Association (APA)
Kiprijanovska, Sanja& Stavridis, Sotir& Stankov, Oliver& Komina, Selim& Petrusevska, Gordana& Polenakovic, Momir…[et al.]. 2014. Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGEMS. International Journal of Proteomics،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-483655
Modern Language Association (MLA)
Kiprijanovska, Sanja…[et al.]. Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGEMS. International Journal of Proteomics No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-483655
American Medical Association (AMA)
Kiprijanovska, Sanja& Stavridis, Sotir& Stankov, Oliver& Komina, Selim& Petrusevska, Gordana& Polenakovic, Momir…[et al.]. Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGEMS. International Journal of Proteomics. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-483655
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483655