Optimization of Human Corneal Endothelial Cells for Culture : The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation
Joint Authors
Balehosur, Deepashree
Toh, Kah-Peng
Mehta, Jodhbir S.
Wu, Fei-Yi
Peh, Gary S. L.
Lee, Man-Xin
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The culture of human corneal endothelial cells (CECs) is critical for the development of suitable graft alternative on biodegradable material, specifically for endothelial keratoplasty, which can potentially alleviate the global shortage of transplant-grade donor corneas available.
However, the propagation of slow proliferative CECs in vitro can be hindered by rapid growing stromal corneal fibroblasts (CSFs) that may be coisolated in some cases.
The purpose of this study was to evaluate a strategy using magnetic cell separation (MACS) technique to deplete the contaminating CSFs from CEC cultures using antifibroblast magnetic microbeads.
Separated “labeled” and “flow-through” cell fractions were collected separately, cultured, and morphologically assessed.
Cells from the “flow-through” fraction displayed compact polygonal morphology and expressed Na+/K+ATPase indicative of corneal endothelial cells, whilst cells from the “labeled” fraction were mostly elongated and fibroblastic.
A separation efficacy of 96.88% was observed.
Hence, MACS technique can be useful in the depletion of contaminating CSFs from within a culture of CECs.
American Psychological Association (APA)
Peh, Gary S. L.& Lee, Man-Xin& Wu, Fei-Yi& Toh, Kah-Peng& Balehosur, Deepashree& Mehta, Jodhbir S.. 2012. Optimization of Human Corneal Endothelial Cells for Culture : The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484082
Modern Language Association (MLA)
Peh, Gary S. L.…[et al.]. Optimization of Human Corneal Endothelial Cells for Culture : The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation. International Journal of Biomaterials No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-484082
American Medical Association (AMA)
Peh, Gary S. L.& Lee, Man-Xin& Wu, Fei-Yi& Toh, Kah-Peng& Balehosur, Deepashree& Mehta, Jodhbir S.. Optimization of Human Corneal Endothelial Cells for Culture : The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484082