Aggregates, Crystals, Gels, and Amyloids : Intracellular and Extracellular Phenotypes at the Crossroads of Immunoglobulin Physicochemical Property and Cell Physiology
Author
Source
International Journal of Cell Biology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-05
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
Recombinant immunoglobulins comprise an important class of human therapeutics.
Although specific immunoglobulins can be purposefully raised against desired antigen targets by various methods, identifying an immunoglobulin clone that simultaneously possesses potent therapeutic activities and desirable manufacturing-related attributes often turns out to be challenging.
The variable domains of individual immunoglobulins primarily define the unique antigen specificities and binding affinities inherent to each clone.
The primary sequence of the variable domains also specifies the unique physicochemical properties that modulate various aspects of individual immunoglobulin life cycle, starting from the biosynthetic steps in the endoplasmic reticulum, secretory pathway trafficking, secretion, and the fate in the extracellular space and in the endosome-lysosome system.
Because of the diverse repertoire of immunoglobulin physicochemical properties, some immunoglobulin clones’ intrinsic properties may manifest as intriguing cellular phenotypes, unusual solution behaviors, and serious pathologic outcomes that are of scientific and clinical importance.
To gain renewed insights into identifying manufacturable therapeutic antibodies, this paper catalogs important intracellular and extracellular phenotypes induced by various subsets of immunoglobulin clones occupying different niches of diverse physicochemical repertoire space.
Both intrinsic and extrinsic factors that make certain immunoglobulin clones desirable or undesirable for large-scale manufacturing and therapeutic use are summarized.
American Psychological Association (APA)
Hasegawa, Haruki. 2013. Aggregates, Crystals, Gels, and Amyloids : Intracellular and Extracellular Phenotypes at the Crossroads of Immunoglobulin Physicochemical Property and Cell Physiology. International Journal of Cell Biology،Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-484428
Modern Language Association (MLA)
Hasegawa, Haruki. Aggregates, Crystals, Gels, and Amyloids : Intracellular and Extracellular Phenotypes at the Crossroads of Immunoglobulin Physicochemical Property and Cell Physiology. International Journal of Cell Biology No. 2013 (2013), pp.1-22.
https://search.emarefa.net/detail/BIM-484428
American Medical Association (AMA)
Hasegawa, Haruki. Aggregates, Crystals, Gels, and Amyloids : Intracellular and Extracellular Phenotypes at the Crossroads of Immunoglobulin Physicochemical Property and Cell Physiology. International Journal of Cell Biology. 2013. Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-484428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484428