Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation
Joint Authors
Bhusari, Vidhya K.
Dhaneshwar, Sunil R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Atenolol and Aspirin as the bulk drug and in tablet dosage forms.
Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of n-butanol : water : acetic acid (8 : 2 : 0.2 v/v/v).
Densitometric evaluation of the separated zones was performed at 235 nm.
The two drugs were satisfactorily resolved with RF values 0.23±0.02 and 0.72±0.02 for Atenolol and Aspirin, respectively.
The accuracy and reliability of the method was assessed by evaluation of linearity (100–600 ng/spot for Atenolol and Aspirin), precision (intraday % RSD was 0.48–1.03 and interday % RSD was 0.68–1.14 for Atenolol, and intraday % RSD was 0.61–1.03 and interday % RSD was 0.69–1.04 for Aspirin), accuracy (100.17±0.32 for Atenolol and 99.73±0.51 for Aspirin), and specificity in accordance with ICH guidelines.
American Psychological Association (APA)
Bhusari, Vidhya K.& Dhaneshwar, Sunil R.. 2012. Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation. ISRN Analytical Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-484877
Modern Language Association (MLA)
Bhusari, Vidhya K.& Dhaneshwar, Sunil R.. Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation. ISRN Analytical Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-484877
American Medical Association (AMA)
Bhusari, Vidhya K.& Dhaneshwar, Sunil R.. Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation. ISRN Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-484877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484877