Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein
Joint Authors
Mirzaei, Maryam
Elias, Cynthia B.
Prakash, Satya
Xu, Yan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-10-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human interleukin-7 (hIL-7) is a cytokine secreted by the stromal cells of the red marrow.
It is important for proliferation during certain stages of B-cell maturation and for T and NK cell survival, development, and homeostasis.
It is a critical growth factor for enhancement and recovery of the immune T-cell.
Because of its strong immunomodulatory effects, hIL-7 may become a valuable supplementary agent for immunotherapeutical treatments in patients with HIV infection or immunodeficiency.
Human IL-7 has previously been produced in various protein expression systems.
In this paper, we present an alternative expression system, in Spodoptera frugiperda cells, for the production of hIL-7 using nonlytic vector systems.
This system allows generation of correctly translated and accurately processed heterologous proteins as soluble recombinant proteins.
Here we report plasmid construction, transfection, and consequent expression of hIL-7 using this nonlytic insect cell expression system.
The levels of secreted hIL-7 in a small scale experiment reached a level of 1.7 μg·1−1 under serum-free cell culture conditions.
American Psychological Association (APA)
Mirzaei, Maryam& Xu, Yan& Elias, Cynthia B.& Prakash, Satya. 2008. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487175
Modern Language Association (MLA)
Mirzaei, Maryam…[et al.]. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-487175
American Medical Association (AMA)
Mirzaei, Maryam& Xu, Yan& Elias, Cynthia B.& Prakash, Satya. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology. 2008. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487175