Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine
Joint Authors
Aldoshin, Sergey
Lyssenko, Konstantin
Kabachkov, Evgeniy
Psikha, Boris
Shkondina, Natal’ja
Syrtsova, Lidia
Sanina, Natalia
Pokidova, Olesia
Kotelnikov, Alexander
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This paper describes a comparative study of the decomposition of two nitrosyl iron complexes (NICs) with penicillamine thiolic ligands [Fe2(SC5H11NO2)2(NO)4]SO4·5H2O (I) and glutathione- (GSH-) ligands [Fe2(SC10H17N3O6)2(NO)4]SO4·2H2O (II), which spontaneously evolve to NO in aqueous medium.
NO formation was measured by a sensor electrode and by spectrophotometric methods by measuring the formation of a hemoglobin- (Hb-) NO complex.
The NO evolution reaction rate from (I) k1 = (4.6 ± 0.1)·10−3 s−1 and the elimination rate constant of the penicillamine ligand k2 = (1.8 ± 0.2)·10−3 s−1 at 25°C in 0.05 M phosphate buffer, pH 7.0, was calculated using kinetic modeling based on the experimental data.
Both reactions are reversible.
Spectrophotometry and mass-spectrometry methods have firmly shown that the penicillamine ligand is exchanged for GS− during decomposition of 1.5·10−4 M (I) in the presence of 10−3 M GSH, with 76% yield in 24 h.
As has been established, such behaviour is caused by the resistance of (II) to decomposition due to the higher affinity of iron to GSH in the complex.
The discovered reaction may impede S-glutathionylation of the essential enzyme systems in the presence of (I) and is important for metabolism of NIC, connected with its antitumor activity.
American Psychological Association (APA)
Syrtsova, Lidia& Sanina, Natalia& Lyssenko, Konstantin& Kabachkov, Evgeniy& Psikha, Boris& Shkondina, Natal’ja…[et al.]. 2014. Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine. Bioinorganic Chemistry and Applications،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-487511
Modern Language Association (MLA)
Syrtsova, Lidia…[et al.]. Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine. Bioinorganic Chemistry and Applications No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-487511
American Medical Association (AMA)
Syrtsova, Lidia& Sanina, Natalia& Lyssenko, Konstantin& Kabachkov, Evgeniy& Psikha, Boris& Shkondina, Natal’ja…[et al.]. Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine. Bioinorganic Chemistry and Applications. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-487511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487511