Image Segmentation and Identification of Paired Antibodies in Breast Tissue
Joint Authors
Simonsson, Martin
Bengtsson, Ewert
Azar, Jimmy C.
Hast, Anders
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Comparing staining patterns of paired antibodies designed towards a specific protein but toward different epitopes of the protein provides quality control over the binding and the antibodies’ ability to identify the target protein correctly and exclusively.
We present a method for automated quantification of immunostaining patterns for antibodies in breast tissue using the Human Protein Atlas database.
In such tissue, dark brown dye 3,3′-diaminobenzidine is used as an antibody-specific stain whereas the blue dye hematoxylin is used as a counterstain.
The proposed method is based on clustering and relative scaling of features following principal component analysis.
Our method is able (1) to accurately segment and identify staining patterns and quantify the amount of staining and (2) to detect paired antibodies by correlating the segmentation results among different cases.
Moreover, the method is simple, operating in a low-dimensional feature space, and computationally efficient which makes it suitable for high-throughput processing of tissue microarrays.
American Psychological Association (APA)
Azar, Jimmy C.& Simonsson, Martin& Bengtsson, Ewert& Hast, Anders. 2014. Image Segmentation and Identification of Paired Antibodies in Breast Tissue. Computational and Mathematical Methods in Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487928
Modern Language Association (MLA)
Azar, Jimmy C.…[et al.]. Image Segmentation and Identification of Paired Antibodies in Breast Tissue. Computational and Mathematical Methods in Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-487928
American Medical Association (AMA)
Azar, Jimmy C.& Simonsson, Martin& Bengtsson, Ewert& Hast, Anders. Image Segmentation and Identification of Paired Antibodies in Breast Tissue. Computational and Mathematical Methods in Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487928