Phenotypic Detection of Metallo-β-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa
Joint Authors
Vadivelu, Jamuna
Chua, Eng Guan
Tay, Sun Tee
Khosravi, Yalda
Loke, Mun Fai
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection.
However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is of global concern.
In this study, 90 imipenem- (IPM- or IP-) resistant P.
aeruginosa (IRPA) isolates, including 32 previously tested positive and genotyped for MBL genes by PCR, were subjected to double-disk synergy test (DDST), combined disk test (CDT), and imipenem/imipenem-inhibitor (IP/IPI) E-test to evaluate their MBLs detection capability.
All three methods were shown to have a sensitivity of 100%.
However, DDST was the most specific of the three (96.6%), followed by IP/IPI E-test interpreted based on the single criteria of IP/IPI ≥8 as positive (62.1%), and CDT was the least specific (43.1%).
Based on the data from this evaluation, we propose that only IRPA with IP MIC >16 μg/mL and IP/IPI ≥8 by IP/IPI E-test should be taken as positive for MBL activity.
With the new dual interpretation criteria, the MBL IP/IPI E-test was shown to achieve 100% sensitivity as well as specificity for the IRPA in this study.
Therefore, the IP/IPI E-test is a viable alternative phenotypic assay to detect MBL production in IRPA in our population in circumstances where PCR detection is not a feasible option.
American Psychological Association (APA)
Khosravi, Yalda& Loke, Mun Fai& Chua, Eng Guan& Tay, Sun Tee& Vadivelu, Jamuna. 2012. Phenotypic Detection of Metallo-β-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488676
Modern Language Association (MLA)
Khosravi, Yalda…[et al.]. Phenotypic Detection of Metallo-β-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-488676
American Medical Association (AMA)
Khosravi, Yalda& Loke, Mun Fai& Chua, Eng Guan& Tay, Sun Tee& Vadivelu, Jamuna. Phenotypic Detection of Metallo-β-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488676