Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis
Joint Authors
Kølvraa, Steen
Gonzalez Dosal, Regina
Jensen, Uffe Birk
Kristensen, Peter
Sørensen, Morten Dræby
Jensen, Kim Bak
Christensen, Britta
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial.
Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures.
Identification of NRBCs in blood samples would be possible provided that specific antibodies are available.
Here we have isolated recombinant antibodies using phage display.
From the panel of antibody fragments specifically recognising ε-Hb, one was chosen for further characterization, DAb1.
DAb1 binds to ε-Hb both in Western blots and immunocytochemistry.
Several ε-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS).
To evaluate the sensitivity of the method, K562 cells (which express ε-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.
American Psychological Association (APA)
Sørensen, Morten Dræby& Gonzalez Dosal, Regina& Jensen, Kim Bak& Christensen, Britta& Kølvraa, Steen& Jensen, Uffe Birk…[et al.]. 2009. Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-488979
Modern Language Association (MLA)
Sørensen, Morten Dræby…[et al.]. Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-488979
American Medical Association (AMA)
Sørensen, Morten Dræby& Gonzalez Dosal, Regina& Jensen, Kim Bak& Christensen, Britta& Kølvraa, Steen& Jensen, Uffe Birk…[et al.]. Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-488979
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488979