Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease
Joint Authors
Aukema, Harold M.
Calvet, James P.
Kugita, Masanori
Nagao, Shizuko
Hiki, Yoshiyuki
Wallace, Darren P.
Toyohara, Takafumi
Morita, Miwa
Abe, Takaaki
Yoshihara, Daisuke
Kurahashi, Hiroki
Yamaguchi, Tamio
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Kidneys are enlarged by aberrant proliferation of tubule epithelial cells leading to the formation of numerous cysts, nephron loss, and interstitial fibrosis in polycystic kidney disease (PKD).
Pioglitazone (PIO), a PPAR-γ agonist, decreased cell proliferation, interstitial fibrosis, and inflammation, and ameliorated PKD progression in PCK rats (Am.
J.
Physiol.-Renal, 2011).
To explore genetic mechanisms involved, changes in global gene expression were analyzed.
By Gene Set Enrichment Analysis of 30655 genes, 13 of the top 20 downregulated gene ontology biological process gene sets and six of the top 20 curated gene set canonical pathways identified to be downregulated by PIOtreatment were related to cell cycle and proliferation, including EGF, PDGF and JNK pathways.
Their relevant pathways were identified using the Kyoto Encyclopedia of Gene and Genomes database.
Stearoyl-coenzyme A desaturase 1 is a key enzyme in fatty acid metabolism found in the top 5 genes downregulated by PIO treatment.
Immunohistochemical analysis revealed that the gene product of this enzyme was highly expressed in PCK kidneys and decreased by PIO.
These data show that PIO alters the expression of genes involved in cell cycle progression, cell proliferation, and fatty acid metabolism.
American Psychological Association (APA)
Yoshihara, Daisuke& Kugita, Masanori& Yamaguchi, Tamio& Aukema, Harold M.& Kurahashi, Hiroki& Morita, Miwa…[et al.]. 2012. Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease. PPAR Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-491314
Modern Language Association (MLA)
Yoshihara, Daisuke…[et al.]. Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease. PPAR Research No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-491314
American Medical Association (AMA)
Yoshihara, Daisuke& Kugita, Masanori& Yamaguchi, Tamio& Aukema, Harold M.& Kurahashi, Hiroki& Morita, Miwa…[et al.]. Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease. PPAR Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-491314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491314