Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells
Joint Authors
Thoresen, G. Hege
Storlien, Len
Rhedin, Magdalena
Strömstedt, Maria
Rustan, Arild C.
Nikolić, Nataša
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We investigated the effects of PGC-1α (peroxisome proliferator-activated receptor γ coactivator-1α) overexpression on the oxidative capacity of human skeletal muscle cells ex vivo.
PGC-1α overexpression increased the oxidation rate of palmitic acid and mRNA expression of genes regulating lipid metabolism, mitochondrial biogenesis, and function in human myotubes.
Basal and insulin-stimulated deoxyglucose uptake were decreased, possibly due to upregulation of PDK4 mRNA.
Expression of fast fiber-type gene marker (MHCIIa) was decreased.
Compared to skeletal muscle in vivo, PGC-1α overexpression increased expression of several genes, which were downregulated during the process of cell isolation and culturing.
In conclusion, PGC-1α overexpression increased oxidative capacity of cultured myotubes by improving lipid metabolism, increasing expression of genes involved in regulation of mitochondrial function and biogenesis, and decreasing expression of MHCIIa.
These results suggest that therapies aimed at increasing PGC-1α expression may have utility in treatment of obesity and obesity-related diseases.Erratum of “Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells”dx.doi.org/10.1155/2013/347567
American Psychological Association (APA)
Nikolić, Nataša& Rhedin, Magdalena& Rustan, Arild C.& Storlien, Len& Thoresen, G. Hege& Strömstedt, Maria. 2011. Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-492681
Modern Language Association (MLA)
Nikolić, Nataša…[et al.]. Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells. Biochemistry Research International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-492681
American Medical Association (AMA)
Nikolić, Nataša& Rhedin, Magdalena& Rustan, Arild C.& Storlien, Len& Thoresen, G. Hege& Strömstedt, Maria. Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells. Biochemistry Research International. 2011. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-492681
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492681