Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field
Joint Authors
El-Bialy, Nihal S.
Rageh, Monira M.
EL-Gebaly, Reem H.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The present study aimed to evaluate the association between whole body exposure to extremely low frequency magnetic field (ELF-MF) and genotoxic , cytotoxic hazards in brain and bone marrow cells of newborn rats.
Newborn rats (10 days after delivery) were exposed continuously to 50 Hz, 0.5 mT for 30 days.
The control group was treated as the exposed one with the sole difference that the rats were not exposed to magnetic field.
Comet assay was used to quantify the level of DNA damage in isolated brain cells.
Also bone marrow cells were flushed out to assess micronucleus induction and mitotic index.
Spectrophotometric methods were used to measure the level of malondialdehyde (MDA) and the activity of glutathione (GSH) and superoxide dismutase (SOD).
The results showed a significant increase in the mean tail moment indicating DNA damage in exposed group (P<0.01,0.001,0.0001).
Moreover ELF-MF exposure induced a significant (P<0.01,0.001) four folds increase in the induction of micronucleus and about three folds increase in mitotic index (P<0.0001).
Additionally newborn rats exposed to ELF-MF showed significant higher levels of MDA and SOD (P<0.05).
Meanwhile ELF-MF failed to alter the activity of GSH.
In conclusion, the present study suggests an association between DNA damage and ELF-MF exposure in newborn rats.
American Psychological Association (APA)
Rageh, Monira M.& EL-Gebaly, Reem H.& El-Bialy, Nihal S.. 2012. Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-492859
Modern Language Association (MLA)
Rageh, Monira M.…[et al.]. Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-492859
American Medical Association (AMA)
Rageh, Monira M.& EL-Gebaly, Reem H.& El-Bialy, Nihal S.. Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-492859
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-492859