Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta
Joint Authors
Schmidt, Thomas
Dechyeva, Daryna
Source
International Journal of Plant Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
By comparative multicolor FISH, we have physically mapped small chromosome fragments in the sugar beet addition lines PRO1 and PAT2 and analyzed the distribution of repetitive DNA families in species of the section Procumbentes of the genus Beta.
Six repetitive probes were applied, including genotype-specific probes—satellites pTS4.1, pTS5, and pRp34 and a dispersed repeat pAp4, the telomere (TTTAGGG)n, and the conserved 18S-5.8S-25S rRNA genes.
Pachytene-FISH analysis of the native centromere organization allowed proposing the origin of PRO1 and PAT2 fragments.
Comparative analysis of the repetitive DNA distribution and organization in the wild beet and in the addition lines allowed the development of a physical model of the chromosomal fragments.
Immunostaining revealed that the PRO1 chromosome fragment binds α-tubulin and the serine 10-phosphorylated histone H3 specific for the active centromere.
This is the first experimental detection of the kinetochore proteins in Beta showing their active involvement in chromosome segregation in mitosis.
American Psychological Association (APA)
Dechyeva, Daryna& Schmidt, Thomas. 2009. Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta. International Journal of Plant Genomics،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-493305
Modern Language Association (MLA)
Dechyeva, Daryna& Schmidt, Thomas. Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta. International Journal of Plant Genomics No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-493305
American Medical Association (AMA)
Dechyeva, Daryna& Schmidt, Thomas. Molecular Cytogenetic Mapping of Chromosomal Fragments and Immunostaining of Kinetochore Proteins in Beta. International Journal of Plant Genomics. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-493305
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493305