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Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor
Joint Authors
Cardinal-Watkins, Chantale
Nicell, Jim A.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Many natural and synthetic estrogens are amenable to oxidation through the catalytic action of oxidative enzymes such as the fungal laccase Trametes versicolor.
This study focused on characterizing the conversion of estradiol (E2) using laccase that had been immobilized by covalent bonding onto silica beads contained in a bench-scale continuous-flow packed bed reactor.
Conversion of E2 accomplished in the reactor declined when the temperature of the system was changed from room temperature to just above freezing at pH 5 as a result of a reduced rate of reaction rather than inactivation of the enzyme.
Similarly, conversion increased when the system was brought to warmer temperatures.
E2 conversion increased when the pH of the influent to the immobilized laccase reactor was changed from pH 7 to pH 5, but longer-term experiments showed that the enzyme is more stable at pH 7.
Results also showed that the immobilized laccase maintained its activity when treating a constant supply of aqueous E2 at a low mean residence time over a 12-hour period and when treating a constant supply of aqueous E2 at a high mean residence time over a period of 9 days.
American Psychological Association (APA)
Cardinal-Watkins, Chantale& Nicell, Jim A.. 2011. Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor. Enzyme Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-493612
Modern Language Association (MLA)
Cardinal-Watkins, Chantale& Nicell, Jim A.. Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor. Enzyme Research No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-493612
American Medical Association (AMA)
Cardinal-Watkins, Chantale& Nicell, Jim A.. Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-493612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493612