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Cloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41
Joint Authors
Li, Meiya
Shi, Xianming
Gan, Zhibing
Cui, Yan
Shi, Chunlei
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the ζ-carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends.
The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa.
Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively.
Phylogenetic analysis demonstrated that the ZDS from C.
protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species.
In addition, we also found that the zds gene expression was upregulated in response to light.
American Psychological Association (APA)
Li, Meiya& Gan, Zhibing& Cui, Yan& Shi, Chunlei& Shi, Xianming. 2011. Cloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-494221
Modern Language Association (MLA)
Li, Meiya…[et al.]. Cloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-494221
American Medical Association (AMA)
Li, Meiya& Gan, Zhibing& Cui, Yan& Shi, Chunlei& Shi, Xianming. Cloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-494221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494221